Computational Design of Phosphotriesterase Improves V‐Agent Degradation Efficiency

Organophosphates (OPs) are potent neurotoxins whose current remedies are not very effective. Here we design and characterize variants of the enzyme phosphotriesterase, which can degrade OPs. We report mutations improving catalytic efficiency between 2- and 5-fold and confirm folding and stability of the resulting variants. These findings are a step towards improved OP bioscavengers.

Abstract

Organophosphates (OPs) are a class of neurotoxic acetylcholinesterase inhibitors including widely used pesticides as well as nerve agents such as VX and VR. Current treatment of these toxins relies on reactivating acetylcholinesterase, which remains ineffective. Enzymatic scavengers are of interest for their ability to degrade OPs systemically before they reach their target. Here we describe a library of computationally designed variants of phosphotriesterase (PTE), an enzyme that is known to break down OPs. The mutations G208D, F104A, K77A, A80V, H254G, and I274N broadly improve catalytic efficiency of VX and VR hydrolysis without impacting the structure of the enzyme. The mutation I106 A improves catalysis of VR and L271E abolishes activity, likely due to disruptions of PTE's structure. This study elucidates the importance of these residues and contributes to the design of enzymatic OP scavengers with improved efficiency.

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